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Figure 2 | Biological Procedures Online

Figure 2

From: MicroRNA Expression Profiling by Bead Array Technology in Human Tumor Cell Lines Treated with Interferon-Alpha-2a

Figure 2

Cell line specific microRNA expression Volcano plot (a) display demonstrates the multi-variant biological diversity of microRNA expression in ME-15 or HuH7 cells. The estimated fold-change value (change factor) is plotted on the X-axis against the p value (limma t statistics) in logarithmic scale on the Y-axis. A linear model, using the expression values of untreated ME-15 cells at 4 h as base together with three parameters to estimate differences in time, treatment, or cell line. The top ranked and qPCR measured microRNAs are annotated. b Quantitative PCR validation of microarray data using eight selected microRNAs. Input total RNA came from independent cell cultures. Data are shown as relative cycling times (ΔCT) calculated with endogenous control RNU18 for ME-15 (color-filled bars) and HuH7 (gray). Error bars represent ΔCT ± Δx (standard deviation of biological replicates). ΔΔCT are noted above the bars together with the significance codes for t statistics defined by the intervals '***' < 0.001 ≤ '**' < 0.01 ≤ '*' < 0.05 ≤ '.' < 0.1.

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