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Table 2 Characteristics of the MRED restriction enzymes used for RARB2

From: A Modified Protocol for Bisulfite Genomic Sequencing of Difficult Samples

RE

Site

No. of non-CpG-C within RE siteA

No. of sites within sequenceB

A*B

AluI

AGC T

1

3

3

AvaI

CC CG GG

2

1

2

DdeI

C TNAG

1

1

1

HhaI

GCG C

1

3

3

HpaII

C CG G

1

3

3

Total

   

12

  1. CpGs are underlined. Bisulfite-convertible cytosines are in boldface. Please note that these cytosines are thymidines in the PCR-amplified product representing bisulfite converted DNA and are therefore not recognized by the RE in question