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Figure 1 | Biological Procedures Online

Figure 1

From: A Modified Protocol for Bisulfite Genomic Sequencing of Difficult Samples

Figure 1

RARB2 sequence under analysis. A Unconverted sequence; B converted sequence (all non-CpG-cytosines have been replaced with thymidines). The 541 bp sequence analyzed is comprised between oligonucleotides JP5 and JP7. CG = CpG dinucleotide under investigation (n = 22); T = non-CpG-cytosine converted to thymidine following bisulfite treatment (n = 82); boxes promoter elements; gray-shaded sequences RE sites (please note that these sites are absent in the converted sequence); +1 transcription start site. Direct repeats of the RARE are indicated. The oligonucleotide sequences are underlined and are designed for converted DNA.

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