EM of DASH/Dam1. a Rotary shadowing shows particles roughly 13 nm in size, with irregular shape. b State-of-the-art negative stain coupled with single particle averaging shows a complex internal structure of the elongated particles. The scale bar indicates 100 nm for the unprocessed images. The averaged images on the right show a monomer, dimer, and trimer. These panels are 14 nm wide. The dimer was the predominant species. Left panel (rotary shadowing) reprinted with permission of Miranda et al. . Right panels (negative stain) reprinted with permission of Wang et al. .