Hydrodynamic analysis of the DASH/Dam1 complex. Gel filtration is shown in a and sucrose gradient sedimentation in b. Independent calibration curves using standard proteins are shown in black and green. Dark and light blue show Spc34p in yeast cytoplasmic extract and in the purified recombinant protein. Red and purple show Hsk3p. The proteins were identified and quantitated by Western blot of the fractions, shown in c. The four protein bands eluted together at 1/D = 0.35 × 107, corresponding to R
s = 7.6 nm, and at 7.4 S. Reproduced from Miranda et al.  with permission of the authors.