Figure 2
From: SDS-PAGE-Based Quantitative Assay for Screening of Kidney Stone Disease
Solubility of salt precipitated urinary proteins in distilled water, 0.01 M PBS and TSE buffer. Lane 1: molecular weight markers; lane 2: pellet fraction of distilled water buffer; lane 3: supernatant fraction of distilled water buffer; lane 4: pellet fraction of 0.01 M PBS buffer; lane 5: supernatant fractions of 0.01 M PBS buffer; lane 6: pellet fractions of TSE buffer; and lane 7: supernatant fractions of TSE buffer. Salt-precipitated urinary proteins were dissolved in the indicated buffers. After centrifugation, the recovered proteins in the pellet fractions and supernatant fractions were separated by using SDS-PAGE. The gel was stained with Coomassie blue.