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  1. Methodology

    A Medium-Throughput Single Cell CRISPR-Cas9 Assay to Assess Gene Essentiality

    Target selection for oncology is a crucial step in the successful development of therapeutics. Clustered regularly interspaced short palindromic repeats (CRISPR)-Cas9 editing of specific loci offers an alterna...

    A. R. Grassian, T. M. E. Scales, S. K. Knutson, K. W. Kuntz, N. J. McCarthy, C. E. Lowe, J. D. Moore, R. A. Copeland, H. Keilhack, J. J. Smith, J. A. Wickenden and S. Ribich

    Biological Procedures Online 2015 17:15

    Published on: 14 November 2015

  2. Research

    Mitogen Activated Protein Kinase (MPK) Interacts With Auxin Influx Carrier (OsAux/LAX1) Involved in Auxin Signaling in Plant

    Mitogen activated protein kinases (MPKs) are serine/threonine protein kinases that contain characteristic T-x-Y motif in the activation loop region. MPKs are important signaling molecules involved in diverse s...

    Tapan Kumar Mohanta, Nibedita Mohanta, Pratap Parida and Hanhong Bae

    Biological Procedures Online 2015 17:13

    Published on: 30 October 2015

  3. Review

    The diversity of fungal genome

    The genome size of an organism varies from species to species. The C-value paradox enigma is a very complex puzzle with regards to vast diversity in genome sizes in eukaryotes. Here we reported the detailed ge...

    Tapan Kumar Mohanta and Hanhong Bae

    Biological Procedures Online 2015 17:8

    Published on: 2 April 2015

  4. Methodology

    cDNA synthesis for BCR-ABL1 detection at the MMR level: the importance of using the appropriate kit

    The synthesis of complementary DNA (cDNA) for use in the detection of BCR-ABL1 at the Major Molecular Response (MMR) level is a well-established method used by clinical laboratories world-wide. However, the quali...

    Jianxiang Chi, Chryso Pierides, Andrie Mitsidou, Andri Miltiadou, Petroula Gerasimou and Paul Costeas

    Biological Procedures Online 2015 17:4

    Published on: 9 February 2015

  5. Research

    Evaluation of method performance for oxidative stress biomarkers in urine and biological variations in urine of patients with type 2 diabetes mellitus and diabetic nephropathy

    Oxidative stress biomarkers such as superoxide dismutase (CuZnSOD), catalase (CAT) and malondialdehyde (MDA) play an important role in the pathogenesis or progression of numerous diseases. Data regarding the b...

    Ergul Belge Kurutas, Yakup Gumusalan, Ali Cetinkaya and Ekrem Dogan

    Biological Procedures Online 2015 17:3

    Published on: 2 February 2015

  6. Methodology

    Comparison of Vibratome and Compresstome sectioning of fresh primate lymphoid and genital tissues for in situ MHC-tetramer and immunofluorescence staining

    For decades, the Vibratome served as a standard laboratory resource for sectioning fresh and fixed tissues. In skilled hands, high quality and consistent fresh unfixed tissue sections can be produced using a V...

    Hadia M Abdelaal, Hyeon O Kim, Reece Wagstaff, Ryoko Sawahata, Peter J Southern and Pamela J Skinner

    Biological Procedures Online 2015 17:2

    Published on: 7 January 2015

  7. Review

    Model of Cation Transportation Mediated by High-Affinity Potassium Transporters (HKTs) in Higher Plants

    Trk/Ktr/HKT transporters probably were evolved from simple K+ channels KcsA. HKT transporters, which mediate Na+-uniport or Na+/K+-symport, maintain K+/Na+ homeostasis and increase salinity tolerance, can be clas...

    Yi Su, Weigui Luo, Wanhuang Lin, Liying Ma and Mohammed Hunayun Kabir

    Biological Procedures Online 2015 17:1

    Published on: 6 January 2015

    The Erratum to this article has been published in Biological Procedures Online 2015 17:9

  8. Review

    The functional role of long non-coding RNAs and epigenetics

    Long non-coding RNAs (lncRNAs) are non-protein coding transcripts longer than 200 nucleotides. The post-transcriptional regulation is influenced by these lncRNAs by interfering with the microRNA pathways, invo...

    Jinneng Cao

    Biological Procedures Online 2014 16:42

    Published on: 15 September 2014

  9. Methodology

    A convenient, optimized pipeline for isolation, fluorescence microscopy and molecular analysis of live single cells

    Heterogeneity within cell populations is relevant to the onset and progression of disease, as well as development and maintenance of homeostasis. Analysis and understanding of the roles of heterogeneity in bio...

    Jordan R Yaron, Colleen P Ziegler, Thai H Tran, Honor L Glenn and Deirdre R Meldrum

    Biological Procedures Online 2014 16:9

    Published on: 8 May 2014

  10. Review

    Integration of bioinformatics to biodegradation

    Bioinformatics and biodegradation are two primary scientific fields in applied microbiology and biotechnology. The present review describes development of various bioinformatics tools that may be applied in th...

    Pankaj Kumar Arora and Hanhong Bae

    Biological Procedures Online 2014 16:8

    Published on: 27 April 2014

  11. Methodology

    Real-time vascular mechanosensation through ex vivo artery perfusion

    Cell-based perfusion studies have provided great insight into fluid-sensing mechanisms, such as primary cilia in the renal and vascular systems. However, the intrinsic limitations of in vitro cell culture, such a...

    Rahul M Prasad, Xingjian Jin, Wissam A AbouAlaiwi and Surya M Nauli

    Biological Procedures Online 2014 16:6

    Published on: 31 March 2014

  12. Methodology

    Detection and quantification of extracellular microRNAs in murine biofluids

    MicroRNAs (miRNAs) are short RNA molecules which regulate gene expression in eukaryotic cells, and are abundant and stable in biofluids such as blood serum and plasma. As such, there has been heightened intere...

    Thomas C Roberts, Anna M L Coenen-Stass, Corinne A Betts and Matthew J A Wood

    Biological Procedures Online 2014 16:5

    Published on: 14 March 2014

  13. Short report

    Isolation of T cells from mouse oral tissues

    Utilizing mouse models provides excellent immunological and experimental tools to study oral immune responses. However for functional assays, isolating T lymphocytes from the oral tissues has proved to be chal...

    Pushpa Pandiyan, Natarajan Bhaskaran, Yifan Zhang and Aaron Weinberg

    Biological Procedures Online 2014 16:4

    Published on: 10 March 2014

  14. Methodology

    Generation of a monoclonal antibody against the glycosylphosphatidylinositol-linked protein Rae-1 using genetically engineered tumor cells

    Although genetically engineered cells have been used to generate monoclonal antibodies (mAbs) against numerous proteins, no study has used them to generate mAbs against glycosylphosphatidylinositol (GPI)-ancho...

    Jiemiao Hu, Long T Vien, Xueqing Xia, Laura Bover and Shulin Li

    Biological Procedures Online 2014 16:3

    Published on: 4 February 2014

  15. Reviewer acknowledgement

    Reviewer acknowledgement 2013

    A peer-reviewed journal would not survive without the generous time and insightful comments of the reviewers, whose efforts often go unrecognized. Biological Procedures Online has been blessed by the support of h...

    Daniel Shanahan

    Biological Procedures Online 2014 16:2

    Published on: 20 January 2014

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