A simple and non-radioactive technique to study the effect of monophosphoesters on matrix vesicle-mediated calcification
© Springer 2004
Received: 22 July 2004
Accepted: 3 December 2004
A simple and non-radioactive technique based on O-cresolpthalein complexone assay was developed to study in vitro non-radioactive calcium (40Ca) deposition by isolated matrix vesicles. Using this technique, the effect of various phosphoester substrates including ATP, AMP and β-GP on in vitro MV-calcification was studied. O-cresolpthalein complexone assay with non-radioactive calcium demonstrated that AMP or β-GP were more effective in promoting calcium deposition by isolated MVs than ATP. The application of this nonradioactive technique, which is highly sensitive and simple, would offer a useful alternative approach to the routinely used radiometric biomineralization assay which employs radioactive 45Ca.