Open Access

The use of antisense oligonucleotides in evaluating survivin as a therapeutic target for radiation sensitization in lung cancer

Biological Procedures Online6:610250

DOI: 10.1251/bpo95

Received: 18 June 2004

Accepted: 3 October 2004

Abstract

Elucidating the mechanism of over and under expression of proteins is critical in developing a better understanding of cancer. Multiple techniques are used to examine differential expression of proteins in cells and assess changes in protein expression in response to therapies such as radiation. Reduced expression can be caused by protein inactivation, mRNA instability, or reduced transcription. The following protocol was used to determine the mechanism for the reduced expression of an antiapoptotic factor, survivin, in normal tissues in response to radiation and the defect in cancer cells that prevents this reduction. We also examined ways to overcome survivin over expression in cancer cells in order to sensitize them to radiation. We will focus on the use of antisense oligonucleotides, cell cycle analysis, and luciferase reporter genes.

Indexing terms

Survivin Antisense oligonucleotides Luciferase

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