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Suppression subtractive hybridization coupled with microarray analysis to examine differential expression of genes in virus infected cells

Biological Procedures Online volume 6, pages 94–104 (2004)Cite this article

Abstract

High throughput detection of differential expression of genes is an efficient means of identifying genes and pathways that may play a role in biological systems under certain experimental conditions. There exist a variety of approaches that could be used to identify groups of genes that change in expression in response to a particular stimulus or environment. We here describe the application of suppression subtractive hybridization (SSH) coupled with cDNA microarray analysis for isolation and identification of chicken transcripts that change in expression on infection of host cells with a paramyxovirus. SSH was used for initial isolation of differentially expressed transcripts, a large-scale validation of which was accomplished by microarray analysis. The data reveals a large group of regulated genes constituting many biochemical pathways that could serve as targets for future investigations to explore their role in paramyxovirus pathogenesis. The detailed methods described herein could be useful and adaptable to any biological system for studying changes in gene expression.

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Authors and Affiliations

  1. Department of Microbiology and Biomedical Genomics Center, University of Minnesota, 1500 Gortner Avenue, 55108, St. Paul, MN

    Shirin Munir, Sushmita Singh, Kuljeet Kaur & Vivek Kapur

Authors
  1. Shirin Munir
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  2. Sushmita Singh
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  3. Kuljeet Kaur
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  4. Vivek Kapur
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Correspondence to Shirin Munir.

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Published: May 26, 2004.

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Munir, S., Singh, S., Kaur, K. et al. Suppression subtractive hybridization coupled with microarray analysis to examine differential expression of genes in virus infected cells. Biol. Proced. Online 6, 94–104 (2004). https://doi.org/10.1251/bpo77

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  • DOI: https://doi.org/10.1251/bpo77

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Biological Procedures Online

ISSN: 1480-9222

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