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Ribosome formation from subunits studied by stopped-flow and rayleigh light scattering

Biological Procedures Online volume 6, pages 35–54 (2004)Cite this article

Abstract

Light scattering and standard stopped-flow techniques were used to monitor rapid association of ribosomal subunits during initiation of eubacterial protein synthesis. The effects of the initiation factors IF1, IF2, IF3 and buffer conditions on subunit association were studied along with the role of GTP in this process. The part of light scattering theory that is essential for kinetic measurements is highlighted in the main text and a more general treatment of Rayleigh scattering from macromolecules is given in an appendix.

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Authors and Affiliations

  1. Department of Cell and Molecular Biology, BMC, Uppsala University, S-75 124, Box 596, Uppsala, Sweden

    Ayman Antoun, Michael Y. Pavlov & Måns Ehrenberg

  2. Institute of Technology, Tartu University, Riia 23, 51010, Tartu, Estonia

    Tanel Tenson

Authors
  1. Ayman Antoun
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  2. Michael Y. Pavlov
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  3. Tanel Tenson
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  4. Måns Ehrenberg
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Correspondence to Måns Ehrenberg.

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Published: March 19, 2004.

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Antoun, A., Pavlov, M.Y., Tenson, T. et al. Ribosome formation from subunits studied by stopped-flow and rayleigh light scattering. Biol. Proced. Online 6, 35–54 (2004). https://doi.org/10.1251/bpo71

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  • DOI: https://doi.org/10.1251/bpo71

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Biological Procedures Online

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