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T cell integrin overexpression as a model of murine autoimmunity

Biological Procedures Online volume 5, pages 211–221 (2003)Cite this article

Abstract

Integrin adhesion molecules have important adhesion and signaling functions. They also play a central role in the pathogenesis of many autoimmune diseases. Over the past few years we have described a T cell adoptive transfer model to investigate the role of T cell integrin adhesion molecules in the development of autoimmunity. This report summarizes the methods we used in establishing this murine model. By treating murine CD4+ T cells with DNA hypomethylating agents and by transfection we were able to test thein vitro effects of integrin overexpression on T cell autoreactive proliferation, cytotoxicity, adhesion and trafficking. Furthermore, we showed that the ability to inducein vivo autoimmunity may be unique to the integrin lymphocyte function associated antigen-1 (LFA-1).

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Authors and Affiliations

  1. Department of Internal Medicine, University of Michigan, 48109-0940, Ann Arbor, MI, USA

    Donna Ray, Ru Ran Mo & Jun Chen

  2. Divisions of Geriatrics and Rheumatology, Department of Internal Medicine, University of Michigan, 1500 East Medical Center Drive, 48109-0940, Ann Arbor, MI, USA

    Raymond L. Yung

Authors
  1. Raymond L. Yung
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  2. Donna Ray
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Correspondence to Raymond L. Yung.

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Published: October 24, 2003

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Yung, R.L., Ray, D., Mo, R.R. et al. T cell integrin overexpression as a model of murine autoimmunity. Biol. Proced. Online 5, 211–221 (2003). https://doi.org/10.1251/bpo64

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  • DOI: https://doi.org/10.1251/bpo64

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