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Progressive rearrangement of telomeric sequences added to both the ITR ends of the yeast linear pGKL plasmid

Biological Procedures Online volume 5, pages 29–42 (2003)Cite this article

Abstract

Relocation into the nucleus of the yeast cytoplasmic linear plasmids was studied using a monitor plasmid pCLU1. InSaccharomyces cerevisiae, the nuclearly-relocated pCLU1 replicated in a linear form (termed pTLU-type plasmid) which carried the host telomeric repeats TG1–3 of 300–350 bp at both ends. The telomere sequences mainly consisted of a major motif TGTGTGGGTGTGG which was complementary to part of the RNA template of yeast telomerase and were directly added to the very end of the pCLU1-terminal element ITR (inverted terminal repeat), suggesting that the ITR end played a role as a substrate of telomerase. The telomere sequences varied among isolated pTLU-type plasmids, but the TG1–3 organization was symmetrically identical on both ends of any one plasmid. During cell growth under non-selective condition, the telomeric repeat sequences were progressively rearranged on one side, but not on the opposite side of pTLU plasmid ends. This indicates that the mode of telomeric DNA replication or repair differed between both ends. Clonal analysis showed that the intense rearrangement of telomeric DNA was closely associated with extreme instability of pTLU plasmids.

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Authors and Affiliations

  1. Molecular Genetics, Applied Microbial Technology, Sojo University, Ikeda 4-22-1, 860-0082, Kumamoto, Japan

    Norio Gunge & Kohsai Fukuda

  2. Department of Geriatric Research, National Institute for Longevity Sciences, Gengo 36-3, 474-8522, Morioka, Obu, Japan

    Hideki Takata & Akira Matsuura

Authors
  1. Norio Gunge
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  2. Hideki Takata
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  3. Akira Matsuura
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  4. Kohsai Fukuda
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Correspondence to Norio Gunge.

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Published: February 17, 2003

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Gunge, N., Takata, H., Matsuura, A. et al. Progressive rearrangement of telomeric sequences added to both the ITR ends of the yeast linear pGKL plasmid. Biol. Proced. Online 5, 29–42 (2003). https://doi.org/10.1251/bpo44

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  • DOI: https://doi.org/10.1251/bpo44

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