Open Access

Mouse aortic ring assay: A new approach of the molecular genetics of angiogenesis

  • Véronique Masson1,
  • Laetitia Devy1,
  • Christine Grignet-Debrus1,
  • Sarah Bernt1,
  • Khalid Bajou1,
  • Silvia Blacher1,
  • Guy Roland1,
  • Yawen Chang2,
  • Timothy Fong2,
  • Peter Carmeliet3,
  • Jean-Michel Foidart1 and
  • Agnès Noël1Email author
Biological Procedures Online4:24

DOI: 10.1251/bpo30

Received: 29 May 2002

Accepted: 17 July 2002

Abstract

Angiogenesis, a key step in many physiological and pathological processes, involves proteolysis of the extracellular matrix. To study the role of two enzymatic families, serine-proteases and matrix metalloproteases in angiogenesis, we have adapted to the mouse, the aortic ring assay initially developed in the rat. The use of deficient mice allowed us to demonstrate that PAI-1 is essential for angiogenesis while the absence of an MMP, MMP-11, did not affect vessel sprouting. We report here that this model is attractive to elucidate the cellular and molecular mechanisms of angiogenesis, to identify, characterise or screen “pro- or anti-angiogenic agents that could be used for the treatment of angiogenesis-dependent diseases. Approaches include using recombinant proteins, synthetic molecules and adenovirus-mediated gene transfer.

Indexing terms

Angiogenesis factor endothelium transgenic mice recombinant adenovirus

Advertisement