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Coupling optical and electrical measurements in artificial membranes: Lateral diffusion of lipids and channel forming peptides in planar bilayers

Biological Procedures Online volume 1, pages 81–91 (1998)Cite this article

Abstract

Planar lipid bilayers (PLB) were prepared by the Montal-Mueller technique in a FRAP system designed to simultaneously measure conductivity across, and lateral diffusion of, the bilayer. In the first stage of the project the FRAP system was used to characterise the lateral dynamics of bilayer lipids with regards to phospholipid composition (headgroup, chain unsaturation etc.), presence of cholesterol and the effect of divalent cations on negatively-charged bilayers. In the second stage of the project, lateral diffusion of two fluorescently-labelled voltage-dependent pore-forming peptides (alamethicin and S4s from Shaker K+ channel) was determined at rest and in the conducting state. This study demonstrates the feasibility of such experiments with PLBs, amenable to physical constraints, and thus offers new opportunities for systematic studies of structure-function relationships in membrane-associating molecules.

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Author notes

  1. Lea E.

    Present address: The Johnson Research Foundation for Molecular Biophysics and Structural Biology, Department of Biochemistry and Biophysics, University of Pennsylvania School of Medicine, 19104-6059, Philadelphia, PA

Authors and Affiliations

  1. UMR 6522 CNRS-Université de Rouen, Bd M. de Broglie, 76821, Mont-Saint-Aignan, France

    Duclohier H. & Helluin O.

  2. School of Biological Sciences, University of East Anglia, NR4 7TJ, Norwich, England

    Lea E.

  3. Institute of Food Research, BBSRC, Colney Research Park, NR4 7UA, Norwich, England

    Mackie A. R. & S. Ladha

Authors
  1. Duclohier H.
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  2. Helluin O.
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  3. Lea E.
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  4. Mackie A. R.
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  5. S. Ladha
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Correspondence to Duclohier H. or S. Ladha.

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Duclohier, H., Helluin, O., Lea, E. et al. Coupling optical and electrical measurements in artificial membranes: Lateral diffusion of lipids and channel forming peptides in planar bilayers. Biol Proced Online 1, 81–91 (1998). https://doi.org/10.1251/bpo10

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Biological Procedures Online

ISSN: 1480-9222

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