Open Access

Yeast exoglycoproteins produced under NaCl-stress conditions as efficient cryoprotective agents

Biological Procedures Online1:10

DOI: 10.1251/bpo1

Abstract

Six extracellular yeast glycoproteins were prepared from three yeast species in osmotic equilibrium and unequilibrium environments and used as non-penetrating cryoadditives. Glycoproteins secreted by the strain Dipodascus australiensis into growth medium containing NaCl (8% w/v) were found to be the most effective cryoadditives. It was possible to use these glycoproteins alone (without DMSO as penetrating agent) for the cryoprotection of the studied yeasts.

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