Fig. 6

Replication, cytotoxicity of PD-SK-375TS in Colo320 cells and its sensitivity to miR-375. (A) Relative expression level of miR-375 in Colo320 cells and murine organs. Expression levels were determined by quantitative RT-PCR. Each miR expression level was normalized against the level of endogenous U6 snRNA expression and is shown relative to miR-375 levels of the pancreas which was set to 1. (B) Virus growth kinetics. Colo320 cells were infected with PD-H, PD-H-375TS, PD-SK or PD-SK-375TS at MOI 0.1. Virus titer was measured by plaque assay at indicated time points. Shown are mean values ± SEM. Significance, * p < 0.05; n.s., not significant. (C) Cell viability. Colo320 cells were infected with PD-H, PD-H-375TS, PD-SK or PD-SK-375 at the indicated MOI. Cell viability was determined by XTT assay 48 h after infection. Shown are mean values ± SEM. Significance, ** p < 0.01; *** p < 0.001; **** p < 0.0001. (D) Silencing of PD-SK-375TS by miR-375. HEK293T cells were transfected with pCMV-miR-216a or pCMV-miR-375 and infected 48 h later with MOI 0.01 PD-SK-375TS. Virus Virus titers were determined by plaque assay 24 h post infection. Shown are mean values ± SEM. Significance, *** p < 0.001