Fig. 2
From: A novel method to efficiently differentiate human osteoclasts from blood-derived monocytes
Macrophages from Teflon bags and cell culture plates do not differ significantly in their gene and marker expression profile. A + B, Flow cytometry: Expression of common macrophage surface marker proteins on cells differentiated either in regular polystyrene cell culture plates or Teflon bags from matched donors. Shown is one representative histogram per marker (A) and the corresponding quantification (B). Blue: antibody-of-interest, grey: isotype control. C RNA-Seq: Volcano plot comparing the gene expression profile in macrophages differentiated in Teflon bags and polystyrene cell culture plates. Black dots denote genes with no significant changes. None of the genes reached the significance threshold at p = 0.05 [–log10 (1.30)]