Table 1 Effect of the experimental treatments on functional parameters of sperm cells and extracellular ROS levels

HTF + 0.4%BSA

78.33 ± 0.88^a

89 ± 0.58^a

5 ± 0.45^a

415 ± 35^a

c-TYH + 0mM MBCD

77.67 ± 1.45^a

79.67 ± 0.88^b

4.5 ± 0.5^a

181 ± 19^a

c-TYH + 0.75mM MBCD

73.67 ± 1.86^a

76.33 ± 1.45^b

12.33 ± 1.2^b

945 ± 45^b

c-TYH + .75mM MBCD + 20ng/ul pCAG-GFP

76.33 ± 2.03^a

78 ± 1.16^b

12.67 ± 0.88^b

930 ± 30^b

c-TYH + .75mM MBCD + 20ng/ul pgRNA-Cas9

77 ± 1.53^a

78 ± 0.58^b

12 ± 1.23^b

950 ± 50^b

c-TYH + 1mM MBCD

71.33 ± 1.33^a

74 ± 0.58^b

15 ± 0.71^b

1189 ± 10.5^b

c-TYH + 1mM MBCD + 20ng/ul pCAG-GFP

73 ± 1^a

75 ± 0.58^b

16.33 ± 1.2^b

1160 ± 15^b

c-TYH + 1mM MBCD + 20ng/ul pgRNA-Cas9

73.33 ± 1.2^a

75.67 ± 0.88^b

15.67 ± 1.33^b

1201.6 ± 20^b

c-TYH + 2mM MBCD

51 ± 1.53^b

53.33 ± 1.76^c

23 ± 1.08^c

3319.5 ± 19.5^c

c-TYH + 2mM MBCD + 20ng/ul pCAG-GFP

51 ± 0.58^b

53 ± 0.33^c

21.67 ± 0.33^c

3330.2 ± 17^c

c-TYH + 2mM MBCD + 20ng/ul pgRNA-Cas9

50.33 ± 0.33^b

53 ± 0.58^c

21.33 ± 0.88^c

3350 ± 10^c

HTF + 0.4%BSA + 3%DMSO + 20ng/ul pCAG-GFP

11.67 ± 0.88^c

44 ± 0.33^d

20.33 ± 0.33^c

3400 ± 25^c

HTF + 0.4% BSA + 3% DMSO + 20ng/ul pgRNA-Cas9

12.67 ± 1.45^c

45 ± 2.19^d

22 ± 1^c

3451 ± 49^c