ZIF-8 Nanoparticle: A Valuable Tool for Improving Gene Delivery in Sperm-Mediated Gene Transfer

Table 2 Effect of ZIF-8 on mouse embryo development after in vitro fertilization

	Oocyte no^o	Fertilization (%)	Four cell Stage (%)	Compact (%)	Blastocyst (%)	GDF Positive (%)
Control	96	89.05 ± 2.07 ^a	89.05 ± 2.07 ^a	87.21 ± 2.71^a	86.56 ± 1.09 ^a	ND
DMSO + Plasmid	89	85.00 ± 5.09 ^a	85.00 ± 5.09 ^a	83.04 ± 2.50 ^a	81.09 ± 0.47 ^a	44.09 ± 1.64 ^a
7.5 µg ml⁻¹	83	95.48 ± 1.10 ^a	85.67 ± 1.98 ^a	82.70 ± 2.58 ^a	81.45 ± 4.57^a	ND
15 µg ml⁻¹	66	92.85 ± 2.12 ^a	92.85 ± 3.68 ^a	92.85 ± 2.12 ^a	90.36 ± 3.88 ^a	ND
30 µg ml⁻¹	86	92.59 ± 4.90 ^a	83.62 ± 0.50 ^a	83.62 ± 0.29 ^a	83.62 ± 0.59 ^a	ND
60 µg ml⁻¹	99	93.93 ± 5.33 ^a	92.31 ± 6.65 ^a	84.83 ± 0.31 ^a	83.33 ± 0.88 ^a	ND
7.5 µg ml⁻¹ + Plasmid CAS9	65	91.87 ± 4.27 ^a	86.58 ± 1.29 ^a	83.27 ± 2.29 ^a	82.15 ± 0.85 ^a	58.87 ± 3.32 ^a
15 µg ml⁻¹ + Plasmid CAS9	73	92.85 ± 2.12 ^a	92.85 ± 2.12 ^a	92.86 ± 2.12 ^a	89.66 ± 2.40 ^a	76.38 ± 3.00 ^b
30 µg ml⁻¹ + Plasmid CAS9	86	92.59 ± 4.90 ^a	83.55 ± 0.33 ^a	83.55 ± 0.33 ^a	81.18 ± 3.11 ^a	83.61 ± 5.19 ^b
60 µg ml⁻¹ + Plasmid CAS9	66	94.04 ± 3.01 ^a	87.08 ± 0.43 ^a	85.08 ± 0.43 ^a	85.08 ± 0.58 ^a	84.96 ± 2.29 ^b

The rates of different stages were calculated by dividing the average values of each stage by the number of two pronuclear (2PN) stages. To determine the fertilization rate, the number of (2PN) cells was divided by the total number of oocytes. (Data are shown as number means ± SEM). (P < 0.05). Significant differences in the same column are indicated by different superscript letters, a, b

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