Table 1 Sperm characteristics determined in male mice while treated with ZIF-8NP and after exposure to plasmid CRISPR/CAS9 (mean ± SEM)

Control

87.33 ± 1.45 ^a

4.33 ± 0.33 ^a

95.00 ± 0.57 ^a

DMSO + Plasmid

43.00 ± 0.55 ^b

21.03 ± 0.27 ^b

7.66 ± 1.45 ^b

7.5 µg ml⁻¹

87 ± 1.73 ^a

4.00 ± 0.57 ^a

84.66 ± 0.33 ^a,c,d,e

15 µg ml⁻¹

86.33 ± 1.20 ^a

3.33 ± 0.33 ^a

74.66 ± 0.33 ^c,d,e

30 µg ml⁻¹

85.33 ± 0.66 ^a

3.33 ± 0.33 ^a

84.00 ± 0.57 ^a,c,d,e

60 µg ml⁻¹

85 ± 1.52 ^a

4.00 ± 0.57 ^a

79.66 ± 0.57 ^d,c,e

7.5 µg ml⁻¹ + Plasmid CAS9

87.33 ± 0.33 ^a

3.333 ± 0.33 ^a

87.4 ± 0.4 ^a

15 µg ml⁻¹ + Plasmid CAS9

85.66 ± 0.66 ^a

4.00 ± 0.57 ^a

75 ± 0.61 ^d,c,e

30 µg ml⁻¹ + Plasmid CAS9

84.33 ± 0.66 ^a

4.33 ± 0.33 ^a

85 ± 0.27 ^a,c,d,e

60 µg ml⁻¹ + Plasmid CAS9

84.00 ± 1.52 ^a

4.66 ± 0.33 ^a

78.2 ± 0.5 ^e,c,d