Fig. 6

Impact of Different ZIF-8 Concentrations on DNA Uptake Efficiency by Mice Sperm b. effect of sperm treatment with various concentrations of ZIF-8 on the efficiency of DNA uptake by mice sperm. Sperm cells were pre-incubated with incubation buffer (CTRL) and 7.5, 15, 30, 60(ug mL⁻¹) and DMSO (3%) for 30 min; after DNA incubation, total DNA was extracted from purified nuclei then, the extracted DNA was analyzed via q-PCR, in accordance with the methodology outlined in the Materials and Methods Section Data are means ± standard deviation of at least two independent experiments performed. P < 0.05 a. Agarose gel electrophoresis of exogenous DNA genomic and ZIF-8NP-DNA complexes: Lanes 1–4, ZIF-8NP/DNA complexes at the concentration of 7.5, 15, 30 and 60 ug ml⁻¹ respectively; Lane 5 represents positive control, lane c⁻ (negative control); L: DNA ladder