Fig. 5

MBD3 promoted EMT in GC cells via activation of the PI3K/AKT pathway. A The expression of EMT proteins was examined in sh-MBD3-MGC-803 and HGC-27 cells by western blot. B The expression of EMT proteins was examined in Flag-MBD3-SGC-7901 and MGC-803 cells by western blot. C Western blotting was used to determine PI3K/AKT pathway protein expression in MGC-803 and HGC-27 cells after transfection with sh-EGFP or sh-MBD3. D Western blotting was used to test PI3K/AKT pathway protein expression in SGC-7901 and MGC-803 cells after transfection with vector or Flag-MBD3