Table 2 Experimental research animal model designs for estimating the critical primary and secondary ischemia times for single-pedicled flaps in male and female subjects at 95% power and planned minimum 3 h time gap between two anesthesia events on the same subject
Animal number per group excluding attrition | Left flap | Right flap | |
|---|---|---|---|
Study Design A | |||
 Group A | Group B |  | |
  7 female | 7 male | No PI | Ligationa |
  7 female | 7 male | PI for 2 h | PI for 8 h |
  7 female | 7 male | PI for 4 h | PI for 10 h |
  7 female | 7 male | PI for 6 h | PI for 12 h |
  N = 28 | N = 28 | n = 7, r = 1 per group | n = 7, r = 1 per group |
Study Design B | |||
 7 female | 7 male | No PI | PI for 2 h |
 7 female | 7 male | PI for 2 h, REP for 6 h, SI for 1 h | PI for 2 h, REP for 6 h, SI for 4 h |
 7 female | 7 male | PI for 2 h, REP for 6 h, SI for 2 h | PI for 2 h, REP for 6 h, SI for 6 h |
 N = 21 | N = 21 | n = 7, r = 1 per group | n = 7, r = 1 per group |
Study Design C | |||
 7 female | 7 male | No PI | PI for 2 h |
 7 female | 7 male | PI for 2 h, REP for 12 h, SI for 1 h | PI for 2 h, REP for 12 h, SI for 5 h |
 7 female | 7 male | PI for 2 h, REP for 12 h, SI for 3 h | PI for 2 h, REP for 12 h, SI for 7 h |
 N = 21 | N = 21 | n = 7, r = 1 per group | n = 7, r = 1 per group |
Study Design D | |||
 7 female | 7 male | No PI | PI for 2 h |
 7 female | 7 male | PI for 2 h, REP for 24 h, SI for 2 h | PI for 2 h, REP for 24 h, SI for 8 h |
 7 female | 7 male | PI for 2 h, REP for 24 h, SI for 4 h | PI for 2 h, REP for 24 h, SI for 10 h |
 7 female | 7 male | PI for 2 h, REP for 24 h, SI for 6 h | PI for 2 h, REP for 24 h, SI for 12 h |
 N = 28 | N = 28 | n = 7, r = 1 per group | n = 7, r = 1 per group |