Fig. 6From: Standardized Pre-clinical Surgical Animal Model Protocol to Investigate the Cellular and Molecular Mechanisms of Ischemic Flap HealingInduction of primary ischemia (PI). A passage underneath each pedicle is made (A-C) large enough for passing B-2 V clamp to occlude both SIEA and SIEV vessels (D). After clamping, the passage is widened to form a ~ 45–60° angle with flap’s base (E)Back to article page