Fig. 10

Flap tissue harvest. A Flap appearance and different surgical outcomes (full survival, partial or complete failure) at chosen end-point. B Flap division into thirds and longitudinal sections. C Removal of sutures and flap excision. D Inspection of pedicle function. D Physical flap division into the proximal (P), middle (M) and distal (D) portions. E Isolation of longitudinal section out of D portion of excised flap for the subsequent fixation and histological (HIS) or immunohistochemical (IHC) analysis