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Table 1 Analytical sensitivity of the assays

From: Development of a Large-Scale Pathogen Screening Test for the Biosafety Evaluation of Canine Mesenchymal Stem Cells

Infectious agent

Limit of detection (copies/reaction)

LOD (%)

Bacteria

Mycoplasma spp.

1

(20/20) 100%

Leptospira interrogans

100

(20/20) 100%

Brucella canis

100

(20/20) 100%

Neorickettsia spp.

10

(20/20) 100%

Rickettsia spp.

10

(20/20) 100%

Borrelia spp.

100

(20/20) 100%

Ehrlichia spp.

10

(20/20) 100%

Bartonella spp.

10

(20/20) 100%

Anaplasma spp.

10

(20/20) 100%

Protozoa

Leishmania spp.

10

(20/20) 100%

Neospora spp.

10

(20/20) 100%

Babesia spp.

100

(20/20) 100%

DNA viruses

CAV-1

10

(19/20) 95%

CaHV-1

10

(20/20) 100%

CPV

10

(20/20) 100%

SuHV-1

100

(20/20) 100%

RNA viruses

CIV

10

(20/20) 100%

CPIV

10

(20/20) 100%

CDV

100

(20/20) 100%

CCoV

10

(19/20) 95%

Rabies virus

10

(20/20) 100%