Fig. 1
Real-time PCR-based evaluation of T7 bacteriophage spike-in control. A Positive amplification of the T7 bacteriophage target sequence using dilution series, B HRM results showing specific melting peaks for the target sequences, which are easily distinguishable from the no template control (NTC) and the dilution that does not show a specific signal (10−9 dilution of T7 phage)