Table 4 Parameters for validation of the method established for quantittaion of target steroid hormones
From: Screening of Natural Compounds for CYP11A1 Stimulation Against Cell Renal Cell Carcinoma
LOD (ng/mL) | LOQ (ng/mL) | Calibration range (µg/mL) | Linearity | Concentration (µg/mL) | Inter day (n = 3) | Intra day (n = 3) | |||
|---|---|---|---|---|---|---|---|---|---|
Accuracy ± SD (%) | Precision (CV%) | Accuracy ± SD (%) | Precision (CV%) | ||||||
Cholesterol | 0.0014 | 0.004 | 0.05—25 | 0.9999 | 1 | 101.2 ± 11.4 | 11.3 | 97 ± 1.9 | 2 |
Pregnenolone | 0.001 | 0.003 | 0.05—5 | 0.9995 | 1 | 93.1 ± 3.7 | 3.9 | 89.5 ± 2.7 | 3 |
0.1 | 104 ± 4.3 | 4.2 | 103.5 ± 4.1 | 3.9 | |||||