Fig. 2

Wound-healing assay. A Caki-1 cells were transfected with CYP11A1 and then treated with 1 and 10 µM of aminoglutethimide (AMG) and mitomycin C (Mito). The wound area at 0 h was set at 100% and was subsequently measured at 12 an 24 h of treatment. Multiple images were collected and analyzed using the ImageJ software. B Histogram showing % of cell-free regions; the significance of differences in areas relative to the corresponding controls is shown (n ≥ 3). **p < 0.01, ***p < 0.001, and ****p < 0.0001