Fig. 8

High HSPB1 expression indicates a poor prognosis in CRC patients. A-B Survival analysis of the HPA TCGA database (n = 596), GSE17536 (n = 177) and GSE14333 (n = 226) CRC datasets revealed that high or low MSI2 expression was not significantly associated with patient outcomes, but patients with high HSPB1 expression had poorer prognosis than those with low HSPB1 expression. C High MSI2 and HSPB1 expression indicates a poorer prognosis than low expression in the HPA TCGA database (n = 596), GSE17536 (n = 177) and GSE14333 (n = 226) CRC datasets. D Representative IHC images of MSI2, HSPB1, p-HSPB1(Ser78) and ACSL4 staining intensity in our clinical CRC specimens. Scale bars, 100 μm. E The positive gene effect Pearson correlation between MSI2 and HSPB1 from DEMETER2 CRC cell lines (R = 0.26). F Heatmap of HSPB1, GPX4, FTH1, PCNA, TFRC and ACSL4 expression from the GDC TCGA COAD database (n = 510). G Pearson correlation analysis from the GDC TCGA COAD database (n = 510) revealed that HSPB1 was positively correlated with the ferroptosis suppressor genes GPX4 (R = 0.53), FTH1 (R = 0.36) and PCNA (R = 0.11) but negatively correlated with the ferroptosis driver genes TFRC (R=-0.32) and ACSL4 (R=-0.36)