Table 2 List of antibodies used in this study for immunolabelling
Antibody | Species raised in | Target | Working Dilutiona | Obtained from |
|---|---|---|---|---|
Orab1 | Rabbit (polyclonal) | NS2 of BTV-1 | 1:2000 | TPIb |
Orab279 | Guinea pig (polyclonal) | BTV-1 structural proteins | 1:2000 | TPIb |
Anti-tubulin | Mouse (monoclonal) | α-tubulin | 1:1000 | Sigma-Aldrich® (T6199) |
AlexaFluor™ 405 | Goat | Mouse IgG (H + L) | 1:250 | Invitrogen™ (A48255) |
AlexaFluor™ 488 | Goat | Guinea pig IgG (H + L) | 1:250 | Invitrogen™ (A-11073) |
AlexaFluor™ 568 | Goat | Rabbit IgG (H + L) | 1:250 | Invitrogen™ (A-11036) |
AlexaFluor™ 568 | Goat | Mouse IgG (H + L) | 1:250 | Invitrogen™ (A-11031) |