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Fig. 7 | Biological Procedures Online

Fig. 7

From: Visualisation of Bluetongue Virus in the Salivary Apparatus of Culicoides Biting Midges Highlights the Accessory Glands as a Primary Arboviral Infection Site

Fig. 7

Bluetongue virus infection in salivary apparatus of a female Culicoides sonorensis following intrathoracic inoculation of BTV. Representative image at 5 dpITI of the salivary apparatus of a female C. sonorensis midge. Image on the left shows one plane taken with differential interference contrast microscopy and cellular tubulin is shown in red (labelling with mouse anti-tubulin, and anti-mouse IgG AlexaFluor™ 568). Image on the right is a maximum projection of at least 50 stack images where cell nuclei are shown in blue (DAPI staining), cellular tubulin is visualised in red (labelling with mouse anti-tubulin, and anti-mouse IgG AlexaFluor™ 568) and BTV viral structural proteins (VSPs) is visualized in green (labelling with in-house antibody Orab279 and anti-guinea pig IgG AlexaFluor™ 488). In both panels, microscope magnification is shown on the top right corner and the scale bar represents 20 µm

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Biological Procedures Online

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