Fig. 5
Bluetongue virus infection in salivary apparatus of females of Culicoides sonorensis infected by blood feeding. Six representative images of infected salivary apparatuses from six different females of C. sonorensis. All images are maximum projections of at least 50 stack images. Midges 1 to 3 are a representative of BTV infection after 8 days post infection (dpi), while midges 4 to 6 are a representative of infection at 15 dpi. In midges 1, 2, 4 and 5 cell nuclei are shown in blue (DAPI staining), cellular tubulin is visualised in red (labelling with mouse anti-tubulin, and anti-mouse IgG AlexaFluor™ 568) and BTV viral structural proteins (VSPs) is visualized in green (labelling with in-house antibody Orab279 and anti-guinea pig IgG AlexaFluor™ 488). Midges 3 and 6 show cellular tubulin in blue (labelling with mouse anti-tubulin, and anti-mouse IgG AlexaFluor™ 405), BTV VSPs in green (labelling with in-house guinea pig antibody Orab279 and anti-guinea pig IgG AlexaFluor™ 488), and BTV non-structural protein 2 (NS2) in red (labelling with in-house rabbit antibody Orab1 and anti-rabbit IgG AlexaFluor™ 568). In all panels, microscope magnification is shown on the top right corner and the scale bar represents 20 µm