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Fig. 4 | Biological Procedures Online

Fig. 4

From: Visualisation of Bluetongue Virus in the Salivary Apparatus of Culicoides Biting Midges Highlights the Accessory Glands as a Primary Arboviral Infection Site

Fig. 4

Salivary glands of males of Culicoides sonorensis and Culicoides nubeculosus. 20X and 40X magnification pictures of the salivary glands of males of C. sonorensis and C. nubeculosus, respectively. The left panels depict gland structures in one z plane by differential interference contrast (DIC) microscopy. In the right panels, gland cellular structures are shown as maximum projection images by fluorescence confocal microscopy, where cell nuclei are shown in blue (DAPI staining) and tubulin is visualised in red (labelling with mouse anti-tubulin, and anti-mouse IgG AlexaFluor™ 568). In all panels, microscope magnification is shown on the top right corner and the scale bar represents 20 µm

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Biological Procedures Online

ISSN: 1480-9222

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