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Fig. 3 | Biological Procedures Online

Fig. 3

From: Visualisation of Bluetongue Virus in the Salivary Apparatus of Culicoides Biting Midges Highlights the Accessory Glands as a Primary Arboviral Infection Site

Fig. 3

3D reconstruction of one salivary gland and associated accessory glands of a female C. sonorensis. Single plane images (z1-z3) show the lumen where cells are absent, surrounded by a single cell layer in both salivary gland and accessory glands. Maximum projection highlights the overall 3D structure. In all panels cell nuclei are shown in blue (DAPI staining) while tubulin is visualised in green (labelling with mouse anti-tubulin, and anti-mouse IgG AlexaFluor™ 488). In all panels, microscope magnification is shown on the top right corner and the scale bar represents 20 µm

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Biological Procedures Online

ISSN: 1480-9222

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