Fig. 2

Salivary apparatus of a Culicoides sonorensis female. A. 20X magnification pictures of two planes (z1 and z2) of a head (H) with a complete salivary apparatus of a female of C. sonorensis, using differential interference contrast (DIC) microscopy. B and C. 40X magnification of the same salivary apparatus as in panel (A). Three different planes (z1 to z3) are shown using both DIC (B) and fluorescence confocal microscopy (C). In B, oval arrows highlight the dilation or ampulla where the salivary and accessory glands merge, and the salivary duct starts (arrow). In C, cell nuclei are shown in blue (DAPI staining) while tubulin is visualised in red (labelling with mouse anti-tubulin, and anti-mouse IgG AlexaFluor™ 568). In both A and B panels, two large salivary glands (a and b) and eight accessory glands (1 to 8) are distinguished. The accessory glands are sac-like shaped and arranged in groups of four as a rosette around each main salivary gland. In all panels, microscope magnification is shown on the top right corner and the scale bar represents 20 µm