Fig. 5

Differential regulation of protein secretion mediated by the ER-Golgi and exosome-pathways by BFA. Human 293T cells were transfected with either SP-gLuc-GFP or Exo-gLuc-GFP for 24 h. The transfected cells were treated with BFA reagent (3 µg/mL) for 24 h. Both conditioned culture medium and transfected cells were collected and subjected to luciferase assay. The secreted luciferase activity in the media from the transfected 293 T cells (A) and luciferase activity in 293T cells (B) were graphed (n = 3, mean ± SD). BFA experiments were also conducted in human HepG2 cells using the same protocol. The secreted luciferase activities in the medium (C), or luciferase activities in transfected cells (D) were graphed (n = 3, mean ± SD). Student’s two-tailed t-test was used to determine the statistical significance, with p-value < 0.05 being considered significant. * P < 0.05; ** P < 0.01