Fig. 1

Configuration and construction of dual-reporters for visualization of secretory pathways in cultured human cells. Schematic illustration of key features of the dual-reporters, depicting dual-reporters of gLuc fused with GFP, under the control of CMV promoter (A). The functional validation of dual-reporters for monitoring of different secretory pathways of either exosome- or ER-Golgi-mediated secretion or non-specific secretion was conducted in 293T cells (B). Both fluorescence and transmitted light images were recorded 48 h after transfection with dual reporters. TLI: transmitted light image. Scale bar: 10 µm