Fig. 4

Characterization of cell type composition. A. Heatmap displaying expression values for astrocytic and neuronal markers. B. Validation of GFAP, S100B, SLC1A3, and MAP2 expression profiles using qPCR. CTRL1, control 1; CTRL2, control 2. For each protocol and cell line, three biological replicates were used. Data are presented as the mean ± SD,*P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001, ***P ≤ 0.0001. C. Heatmap showing the percentage of iPSC-derived cells in astrocytic cultures sharing the expression profile with the cell types identified in Smajic et al. [41]. D. Heatmap showing the percentage of generated astrocytic cultures sharing the expression signatures with the cortical cell types identified in Agarwal et al. [40]. E. Heatmap displaying the comparison of the generated astrocytes with the dataset from human embryonal midbrain at week 8 [42]. CTRL1, control 1; CTRL2, control 2; Mgl, microglia; Astro, astrocytes; Epend, ependymal cells; ODCs, oligodendrocytes; Neur, neurons; OPCs, oligodendrocyte precursor cells; ProgFPL, lateral floorplate progenitor; Rgl1, radial glia-like cell type 1; NbML1, mediolateral neuroblast type 1; OMTN, oculomotor and trochlear nucleus; DA1, dopaminergic neurons 1; NbML5, mediolateral neuroblast type 5; ProgM, midline neuronal progenitor; ProgFPM, medial floorplate progenitor; RN, red nucleus; DA0, dopaminergic neurons 0; NbM, medial neuroblasts; NProg, neuronal progenitor; ProgBP, basal plate progenitor