Fig. 2

Morphological analysis of the generated astrocytes. A. Scheme summarizing the experimental procedure. B. Representative images for the late astrocytic marker GFAP for both protocols. The brightness and contrast for the image of CTRL2 in the SSC protocol was adjusted. C. Quantification of GFAP⁺ cells in astrocytic cultures using high-content imaging. For each marker, protocol and cell line, three biological replicates were used. Data are presented as the mean ± SD. D. Representative images for the early astrocytic marker Vimentin for both protocols. Scale bar: 20 µm. E. Quantification of the mean intensity of the Vimentin signal within the segmented cellular mask. Data are presented as the mean ± SD. F. Assessment of astrocytic morphology in GFAP⁺ cells. The summed area of astrocytic soma was normalized to the summed cell area. The summed area of astrocytic processes was normalized to the summed soma area. The summed area of astrocytic processes was normalized to the summed cell area. The summed perimeter of astrocytic processes was normalized to the summed cell area. *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001, ***P ≤ 0.0001. Scale bar: 20 µm. CTRL1, control 1; CTRL2, control 2; SD, standard deviation. Part of the figure was generated using Biorender