Fig. 1
From: Comparison of two protocols for the generation of iPSC-derived human astrocytes
Generation of the astrocytic cultures in this study. A. Scheme representing the main steps required to obtain astrocytes with the different protocols. B. Overview of the LSF protocol. NDM, neurodifferentiation medium (see materials and methods section for details); ADM, astrodifferentiation medium (see materials and methods section); SB, SB431542; LDN, LDN-193189; CNTF, ciliary neurotrophic factor; BMP4, bone morphogenetic factor 4. C. Overview of the SSC protocol. N2B27, medium comprised of DMEM-F12, neurobasal medium and supplements (see material and methods section), CHIR, CHIR99021; PMA, purmorphamine; AA, ascorbic acid; EGF, Epidermal Growth Factor; bFGF, basic fibroblast growth factor; LIF, leukemia inhibitory factor; FBS, fetal bovine serum; NPCs, neural precursor cells; NSCs, neural stem cells. The figure was generated using Biorender