Fig. 5

FOXP3-mediated regulation promoted GC progression and stemness. A, B Proliferation curves of MGC803 cells transfected with sh-LINC00853 or/and FOXP3 OE and MKN-78 cells with overexpression of LINC00853 or/and sh-FOXP3, as determined by CCK8 assay. C-E Flow cytometry quantitation of transfected MGC803 cells and MKN-78 cells after indicated transfection. F–H Transwell assay was conducted to evaluate the cell migration of transfected MCG803 and MKN-78 cells. Scale bar = 100 μm. I-K Flow cytometry assessment of CD44 positive proportion of transfected cells. (L-M) RT-PCR analysis to identify the expression levels of stemness genes, including OCT4, NANOG, KLF4 and SOX2. *p < 0.05, **p < 0.01