Fig. 4

LINC00853 promoted PDZK1IP1 expression by interacting with transcriptional factor FOXP3. A, B The expression levels of the potential transcription factors predicted by UCSC and PROMO database were validated by RT-PCR after transfection with sh-LINC00853 or LINC00853 OE, and FOXP3 gene expression was observed as most down/up-regulated. C DNA motif of FOXP3 predicted from JASPAR. D The correlation between LINC00853 expression and FOXP3 expression in GC patients, as analyzed in GEPIA database. E The colocalization of LINC00853 and FOXP3 in GC cells was examined via FISH assay. Scale bar = 20 μm. RNA pulldown (F) and RIP assay (G-H) results demonstrating the interaction between LINC00853 and FOXP3. I-K Western Blots assay was carried out to determine the expression of PDZK1IP1 and FOXP3 after indicated transfection. *p < 0.05, **p < 0.01, ns = non-significant