Fig. 3

Alteration of PDZK1IP1 expression reversed LINC00853-mediated progression and stemness in GC cells. A RT-PCR was applied to investigate the level of PDZK1IP1 in MGC803 and MKN-78 cells. B, C The level of PDZK1IP1 was validated via RT-PCR experiment in MGC803 and MKN-78 cells after indicated transfection. D, E Proliferation curves of MGC803 cells transfected with sh-LINC00853 or/and PDZK1IP1 OE and MKN-78 cells with overexpression of LINC00853 or/and sh-PDZK1IP1, as determined by CCK8 assay. F–H Apoptosis assay of transfected MGC803 and MKN-78 cells by flow cytometry analysis of Annexin V-FITC/PI staining. I-K Transwell migration assay of MGC803 and MKN-78 cells following indicated transfection. Scale bar = 100 μm. L-N Flow cytometry assessment of CD44 positive proportion after transfection. O, P RT-PCR analysis of pluripotency genes, OCT4, NANOG, KLF4 and SOX2 in MGC803 and MKN-78 cells. *p < 0.05, **p < 0.01