Fig. 2

LINC00853 enhanced the proliferation, migration and stemness of GC cells. A MGC803 cells were transfected with lentiviruses encoding either the blank vector or LINC00853 shRNA (sh-LINC00853). The interfering efficiency of the lentivirus encoding sh-LINC00853 were confirmed by RT-PCR analysis. B The level of LINC00853 was clarified via RT-PCR after down/ up regulation of LINC00853 in MGC803 and MKN-78 cells, respectively. C, D CCK-8 test was applied to assess cell viability at 0, 48, 72, and 96 h time points. E The apoptosis of MGC803 and MKN-78 cells was investigated via staining with Annexin V/PI, followed by flow cytometry analysis. F Migration ability of cells examined via Transwell assay. Scale bar = 100 μm. G The effect of knockdown or overexpression of LINC00853 on serum-free sphere formation was evaluated using a tumor sphere formation assay. Scale bar = 100 μm. H The CD44 positive proportion was determined by flow cytometry. I, J The levels of stemness-related factors KLF4, SOX2, NANOG, and OCT4 were detected through RT-PCR analysis. *p < 0.05, **p < 0.01