Fig. 1

LINC00853 exhibited high expression in GC and was related to poor prognosis. A Heatmap of the top 100 upregulated or down-regulated differentially expressed lncRNAs from TCGA GC dataset. B The volcano plot of the lncRNAs in The TCGA dataset. C LINC00853 and PDZK1IP1 genomic loci from Ensembl database. D The level of LINC00853 in 366 GC tumor tissues and 359 normal tissues were obtained from TCGA database. E The correlation between LINC00853 expression and lymph node metastasis of GC patients. F The correlation between LINC00853 level and tumor stages of GC patients. G Kaplan–Meier overall survival curves in LINC00853 low and high expression of GC patients. H The level of LINC00853 in GC was examined by in situ hybridization on GC clinical samples. Scale bar = 50 μm. I The level of LINC00853 was clarified via RT-PCR analysis in 4 GC cell lines (SGC7901, AG5, MGC803, MKN-78). GES-1: normal gastric cell. J A FISH test was conducted to examine the cellular localization of LINC00853 in MGC803 and MKN-78 cells. DAPI was applied to localize cell nuclei. Scale bar = 50 μm. *p < 0.05, **p < 0.01, ***p < 0.001