Fig. 2

Bioinformatic analysis for predication of genome safe harbor loci in the chicken genome. A-a The schematic presentation of the validated HIPP locus including its flanking genes in the mouse, human, and pig genomes. A-b The schematic presentation of the validated ROSA locus including its flanking genes in the mouse, human, and pig genomes. A-c, A-d The schematic presentation of the potential cHIPP and cROSA loci in the chicken genome. Flanking genes around the validated HIPP locus (i.e., DRG1/ EIF4ENIF1) have been exactly the same as the genes found around the predicted cHIPP locus in the chicken genome, but the genes surrounding the validated ROSA locus (i.e., THUMPD3/SETD5/SRGAP3) have been relatively the same as the genes seen around the predicted cROSA locus. A-e The schematic presentation of the non-GSH cOVA locus in the chicken genome. B The coordinates of DRG1/EIF4ENIF1, THUMPD3/SRGAP3, and OVAL genes relative to the location of TADs, extracted from the chicken Hi-C data, and visualized by JUICEBOX online software (adopted from ref [48]). C The expression levels of the flanking genes in several tissues and developmental stages, adopted from the Gene Expression Atlas. ⇨: (adopted from ref [49]). ➲: (adopted from ref [50]). TPM avg.: transcripts per million averages. E: embryonic day. PN: post natal day