Fig. 8

Knockdown CLSPN significantly inhibited lung cancer cell proliferation and induced S and G2/M arrest in vitro. A RT-qPCR and western blot confirmed the knockdown efficiency of CLSPN in A549 cells. B The knockdown efficiency of CLSPN in PC9 cells. C The effect of CLSPN on cell viability was confirmed by CCK-8 assays. D EdU assays were used to evaluate cell proliferative ability after knockdown CLSPN (Scare bar = 100 μm). E Colony formation in untreated, NC and sh-CLSPN groups. F Cell cycle analyses were performed by flow cytometry. *P < 0.05, **P < 0.01, ***P < 0.001, ns: no significance