Fig. 7

Identification of CLSPN expression in LUAD tissues and cell lines. A CLSPN mRNA expression in LUAD tissues and adjacent normal lung tissues (n = 28). B Claspin protein levels in paired tissues (n = 8). C CLSPN mRNA levels in 6 LUAD cell lines (H1299, Calu-3, SPCA1, HCC827, PC9 and A549) and human normal pulmonary epithelial cell (Beas2B). D Claspin protein levels in 6 LUAD cell lines and human normal pulmonary epithelial cell. E Representative immunofluorescence staining of Claspin, CD8, PD-1, and PD-L1 in high- and low-Claspin group (Scare bar = 50 μm). *P < 0.05, **P < 0.01, ***P < 0.001